Practical (laboratory) lesson No. 12. ABSORPTION OF LIGHT

The student must learn the material on the topic and be able to answer the following questions:

1. Interaction of light with matter. Light absorption.
2. Bouguer's law
3. The Bouguer-Lambert-Beer law.
4. Optical density of solutions.
5. Absorption spectra of molecules of some biologically important compounds.
6. Determination of the concentration of colored solutions using a photoelectric colorimeter.
7. Application of spectrophotometry in medicine.

Guidelines

1. Study the lecture presentation material. (File attached)
2. Write in the workbook the laboratory protocol as described in the attached file. In a concise protocol theory reflect the answers to the questions on the topic.
3. Get acquainted with the method of determining the concentration of colored solutions using a photoelectric colorimeter (video)
4. Select an individual task from the attached file. Perform calculations. Estimate measurement errors.
5. Complete the lab report and email the instructor a photo of the report.

Literature

Lecture notes.

Classwork

Fulfill laboratory work "Determination of the concentration of a colored solution using a photoelectric colorimeter"
 

Out-of-class work

Lecture
Laboratory work
Error estimation
Individual tasks